INSULIN PLANT

Insulin Plant (Costus Ingneus) Medicinal Herbs
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Insulin plant (COSTUS IGNEUS (OR )COSTUS PICTUS) is a relatively new entrant to Kerala and India. The plant is a late entrant to Kerala Ayurvedic medicinal herb scene mostly from USA. Insulin plant has not got a Malayalam name yet, except the occasional use of insulin chedy or insulin chedi, where chedy means a plant. The catchphrase of this plant is a leaf a day keeps diabetes away
The plant is characterized by large fleshy looking leaves. It grows very quickly. Propagation is by stem cutting. It grows in slightly shady areas.
Diabetes patients are advised to chew down a leaf in the morning and one in the evening for a month. Allopathic doctors too recommend it and it is found to be effective in bringing blood sugar levels under completely under control. There is also dried and ground powder of the leaves now available in the market.
Costus ingneus belongs to the family zingiberaceae.
(Origin -Florida, USA)
A Magic Cure for Diabetes as never before with proven effects.
Santhimadom Agro Farm has secured these plants from the U.S.A and they are being multiplied using stem cuttings.
Dosage for diabetes patients
1. The patient has to take two leaves per day in the morning and evening and in the evenings for one week. The leaves must be chewed well before swallowing.
2. After one week the patient should take one leaf each in the morning and evening.
3. This dosage should be continued for 30 days.
This medicine is being increasingly prescribed by doctors. In 90 % of the cases diabetes has been found to be curable using this medicine. Just try it for yourself.

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pl download from link; http://www.filefactory.com/file/a1beeef/n/insulin.rar

PHARMACOLOGICAL EVALUATION OF COSTUS PICTUS

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Journal of Health Science, 53(6) 655–663 (2007)

Anti-diabetic Activity of Methanol Leaf Extract of
Costus pictus D.D ON in Alloxan-induced Diabetic Rats
 
655 

Nandhakumar Jothivel,∗, a Sethumathi Pudhupalayam Ponnusamy,aMalini Appachi,b
Sengottuvelu Singaravel,aDuraisamy Rasilingam,aKarthikeyan Deivasigamani,a
and Sivakumar Thangavela

aDepartment of Pharmaceutics, Nandha College of Pharmacy, Koorapalaym Pirivu, Pitchandampalayam (P.O), Erode, Tamil Nadu,
638 052, India andbDepartment of Biochemistry, Maharaja College for Women, Post Box No.:17, Perundurai main road, Erode, Tamil
Nadu, 638 052, India

(Received February 9, 2007; Accepted August 24, 2007)

The methanol extract of Costus pictus (C. pictus) D.DON (Family: Zingiberaceae) leaf was investigated for its
anti-diabetic effect in Wistar Albino rats. Diabetes was induced in Albino rats by administration of single doses
of alloxan monohydrate (120 mg/kg, i.p.). The methanol extract of C. pictus (MECP) at a dose of 120 mg/kg, p.o.
was administered as single dose per day to diabetes-induced rats for a period of 21 days. The effect of MECP leaf
extract on blood glucose, plasma insulin, serum lipid profile [cholesterol, triglycerides, phospholipids, very low-
density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL)], serum enzymes
[serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT), alkaline
phosphatase (ALP)], total protein, and liver glycogen were measured in the diabetic rats. Histopathological studies
of liver, pancreas and kidney were also carried out. MECP elicited significant (p <> 250 mg/dl) were selected for the
study.
Experimental Grouping of Animals —— The ex-
perimental rats were divided into four groups of six
animals in each group. Group I, animals served as
normal healthy controls, which received 0.5% w/v
carboxymethylcellulose sodium (CMC). Group II,
untreated diabetic control. Group III, diabetic rats
given methanol leaf extract of C. pictus (120 mg/kg,
p.o) at a single dose per day. The dose (120 mg/kg,
p.o.) was selected on the basis of earlier-reported
toxicity studies on methanol leaf extract of C. pictus
D.DON.18)Group IV, control rats given methanol
leaf extract of C. pictus (120 mg/kg, p.o.) at a sin-
gle dose per day. The extract was administered for a
period of 21 days. Body weight of the animals was
recorded every week.
 
 

No. 6
 




Table 1. Qualitative Phytochemical Analysis in Different Extracts of Leaves of C. pictus Plant
Plant Extractive solvents of
 

657
 
constituents
Carbohydrate
Protein
Steroids
Alkaloids
Tannins
Glycosides
Saponins
Flavonoids
Fixed oils
 
Petroleum ether
−
−
+
−
−
−
−
−
+
 
Chloroform
−
−
+
+
−
−
−
−
−
 
Methanol
+
+
−
+
+
−
+
+
−
 
Water
+
+
−
−
+
+
+
−
−
 
+ve and –ve symbol indicates the presence and absence respectively of plant constituents with respect to
extractive solvents in the increasing order of polarity.
 

Collection of Liver, Pancreas, Kidney, and
Blood —— At the end of the treatment blood was
collected by direct cardiac puncture and serum was
separated by centrifugation at 2500 rpm. The rats
were sacrificed by cervical dislocation and organs
were excised immediately and thoroughly washed
with ice cold physiological saline. The serum col-
lected was used for biochemical estimations.
Estimation of Biochemical Parameters ——
Serum glucose, plasma insulin (estimated by
ELISA method using Boehinger Mannheim Gmbh
kit, Werk Penzberg, Germany), liver glycogen,
serum lipid profile, serum glutamate oxaloacetate
transaminases (SGOT), serum glutamate pyru-
vate transaminases (SGPT), alkaline phosphatase
(ALP), and serum protein content were determined
standard procedures in an auto analyzer using
Ecoline kits (E. Merck, Mumbai, India).
Histopathological Investigation —— Liver, kid-
ney, and pancreas were washed in saline and a small
portion of these organs was quickly fixed in 10%
formalin. Then, the tissues were processed by stan-
dard histopathological technique (i.e. dehydration
through graded isopropyl alcohol, cleaning through
xylene and impregnated in paraffin wax for 2 hr).
Wax blocks were made, sections were used for cut-
ting microtome and stained by haematoxylin eosin
method and photographed.
Statistical Evaluation —— All results are ex-
pressed as mean ± S.D. Statistical evaluation was
done using one-way analysis of variance (ANOVA),
followed by Student’s t-test.


RESULTS AND DISCUSSION

Phytochemical Analysis
Compounds of different polarity from dried
 

leaves of C. pictus were extracted by sequential
extraction process using different solvents such as
petroleum ether, chloroform, methanol, and wa-
ter (Table 1). These sequential extracts were sub-
jected to preliminary phytochemical screening for
the presence of different chemical groups. Of all ex-
tracts tested, methanol extract was found to contain
the highest number of phytochemicals such as car-
bohydrates, triterpenoids, proteins, alkaloids, tan-
nins, saponins, and flavonoids. The pentacyclic
triterpenoids such as α- and β-amyrin and related
compounds occur especially in waxy coatings of the
leaves.19, 20) Mostly the terpenic compounds were
successfully isolated from leaves.21)From the re-
sults of earlier-reported studies, it is known that
triterpene mixture possesses bioactive anti-diabetic
properties.22–26)

Body Weight
Body weight increased significantly (p < n =" 6);" n =" 6);" n =" 6);">